Journal: Molecular Psychiatry

Article Title: SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior

doi: 10.1038/s41380-018-0260-9

Figure Lengend Snippet: Lack of SERT increases the number of functional PFC-to-DRN synapses. a rAAV-CAG-hChR2(H134R)-mCherry was bilaterally injected into the PFC of P4–P5 control or SERT-KO mice. Photograph showing mCherry expression after the PFC AAV injection (upper left). Optogenetic stimulation and electrophysiological patch clamp recordings were made starting at P28 in coronal sections containing the DRN, as shown by the photograph of the immunolabeling of PFC mCherry+ axons innervating to DRN 5-HT neurons, identified by the presence of the enzyme TPH2 (upper right). b Amplitude of optogenetically evoked EPSCs (oEPSCs) at synapses from PFC terminals onto DRN putative 5-HT neurons (left) and non-5-HT neurons (right) at various light stimulation intensities. In control (SERT Cre/+ ) (5-HT: n = 10 cells/5 animals; non-5-HT: n = 7 cells/4 animals); in SERT-KO (SERT Cre/Cre ) (5-HT: n = 10 cells/3 animals; non-5-HT: n = 6 cells/3 animals). Top: example traces at 9.8 mW (black/gray) and at 2 mW (red) stimulation); Bottom: input/output curves. Two-way ANOVA on 9.8 mW intensity: genotype x cell-type interaction (F 1,29 = 0.003, p = 0.95); Genotype main effect (F 1,29 = 9.32, * p < 0.01); Cell-type main effect (F 1,29 = 0.51, p = 0.48). c AMPAR/NMDAR ratios at synapses from PFC-to-DRN 5-HT neurons (left) and non-5-HT neurons (right) in control (5-HT: n = 10 cells/4 animals; non-5-HT: n = 7 cells/3 animals), and SERT-KO (5-HT: n = 11 cells/3 animals; non-5-HT: n = 6 cells; 3 animals). The AMPAR responses were calculated at the peak of −50 mV, whereas NMDAR responses were determined at + 40 mV, 50 ms after stimulation. Top: example traces; bottom: bar graphs. Two-ways ANOVA: Genotype x Cell-type interaction (F 1,30 = 0.007, p = 0.94); Genotype main effect (F 1,30 = 0.16, p = 0.69); Cell-type main effect (F 1,30 = 4.51, p < 0.05). Blue bars indicate blue light stimulation. Error bars represent SEM

Article Snippet: Subsequently, after a few hours of fixation in 4% PFA at 4 °C, brain slices were processed for immunohistochemistry using a rabbit polyclonal antibody against TPH2 (1:2000, Novus Biologicals, NB100-74555) to identify 5-HT neurons.

Techniques: Functional Assay, Injection, Control, Expressing, Patch Clamp, Immunolabeling

Journal: Nutrients

Article Title: Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism

doi: 10.3390/nu14061229

Figure Lengend Snippet: Comparison of IDO and TPH2 immuno-positive MOD, AD and H-score in different brain regions of rats in each group.

Article Snippet: The membranes were sealed with 5% non-fat milk in TBS-T solution and placed at room temperature for 4 h. The membranes were incubated overnight at 4 °C with the following primary antibodies: (1) rabbit anti-TPH1 antibody (1:1000, Boster, Wuhan, Hubei, China); (2) rabbit anti-IDO2 antibody (1:2000, Boster, Wuhan, Hubei, China); (3) rabbit anti-TPH2 antibody (1:1000, Boster, Wuhan, Hubei, China).

Techniques: Comparison

Journal: Nutrients

Article Title: Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism

doi: 10.3390/nu14061229

Figure Lengend Snippet: Expression of TPH2 in prefrontal cortex ( a – c ) and hippocampus ( d – f ) of three groups of rats. ( a , d ) CON group, ( b , e ) GH group, ( c , f ) GL group. DAB × 400.

Article Snippet: The membranes were sealed with 5% non-fat milk in TBS-T solution and placed at room temperature for 4 h. The membranes were incubated overnight at 4 °C with the following primary antibodies: (1) rabbit anti-TPH1 antibody (1:1000, Boster, Wuhan, Hubei, China); (2) rabbit anti-IDO2 antibody (1:2000, Boster, Wuhan, Hubei, China); (3) rabbit anti-TPH2 antibody (1:1000, Boster, Wuhan, Hubei, China).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Physical Weight Loading Induces Expression of Tryptophan Hydroxylase 2 in the Brain Stem

doi: 10.1371/journal.pone.0085095

Figure Lengend Snippet: Primer sequence.

Article Snippet: Primary antibody was rabbit anti-tph2 (1∶200, Thermo Scientific), and secondary antibody was Alexa Fluor® dye-conjugated goat anti-rabbit IgG (diluted 1∶200 in blocking solution, Jackson ImmunoResearch).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Physical Weight Loading Induces Expression of Tryptophan Hydroxylase 2 in the Brain Stem

doi: 10.1371/journal.pone.0085095

Figure Lengend Snippet: (A) mRNA levels of tph2 in the BS with knee loading, tail suspension, and knee loading followed by tail suspension. (B) Immunoblots displaying protein levels of tph2 and ß-actin in the BS of mice with knee loading. Next to immunoblots showing tph2 protein fold change. (C) Relative mRNA abundance of REST and Sim1 along with that of Pet 1, lhx8, and RGS4 in the brain in response to knee loading. Dash lines denote the mRNA levels of sham loaded controls. Note that “K+T” denotes knee loading plus tail suspension.

Article Snippet: Primary antibody was rabbit anti-tph2 (1∶200, Thermo Scientific), and secondary antibody was Alexa Fluor® dye-conjugated goat anti-rabbit IgG (diluted 1∶200 in blocking solution, Jackson ImmunoResearch).

Techniques: Suspension, Western Blot

Journal: PLoS ONE

Article Title: Physical Weight Loading Induces Expression of Tryptophan Hydroxylase 2 in the Brain Stem

doi: 10.1371/journal.pone.0085095

Figure Lengend Snippet: (A) Rectangular region of interest in caudal brain sections. Orientation of medial and dorsal corresponds to micrographs in B. (B) Representative micrographs from bregma −5.9±0.1 mm showing tph2 immunoreactivity in mice treated with sham, knee loading, and tail suspension. Nuclear counterstain by DAPI overlaid with tph2 in the lower panels. (C) The proposed role of knee loading and unloading in serotonin synthesis through tph2 in the brain.

Article Snippet: Primary antibody was rabbit anti-tph2 (1∶200, Thermo Scientific), and secondary antibody was Alexa Fluor® dye-conjugated goat anti-rabbit IgG (diluted 1∶200 in blocking solution, Jackson ImmunoResearch).

Techniques: Suspension